Journal: Cell reports

Article Title: Chronic Corticosterone Elevation Suppresses Adult Hippocampal Neurogenesis by Hyperphosphorylating Huntingtin.

doi: 10.1016/j.celrep.2020.107865

Figure Lengend Snippet: Figure 4. Phosphorylation of HTT at S1181/S1201 Influences Hippocampal Neurogenesis (A–C) Cells counts were performed in the DG of vehicle- and CORT-treated WT and HdhS1181A/S1201A mice (at least 4 for animals per condition) to quantify proliferation (Ki67+ cells, A), survival (BrdU+ cells, B), and immature neurons (DCX+ cells, C). *p < 0.05, **p < 0.01, ***p < 0.001. (D) Representative images show BrdU+ surviving cells (cyan) expressing (or not) the terminal marker for granule cell differentiation calbindin (magenta) on DG sections of WT and HdhS1181A/S1201A mice treated with vehicle or CORT. (E) The histogram shows the quantification of the number of BrdU+ and calbindin+ cells (3 animals per condition). *p <0.05, **p < 0.01. (F and G) Dendritic length (F) and complexity (G) were evaluated in both genotypes and treatment conditions (3 animals per condition). (H) Representative drawings of dendrites of DCX-positive neurons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 comparing the HdhS1181A/S1201A vehicle group with the WT vehicle group, $p < 0.05, $$p < 0.01, $$$p < 0.001, $$$$p < 0.0001 comparing the HdhS1181A/S1201A -CORT group with the WT CORT group. Error bars, SEM. See also Figure S2.

Article Snippet: Slices were rinsed and blocked in 3% normal donkey serum in PBS containing 0.3% Triton X-100 for 1h at room temperature then incubated overnight at room temperature in 1:100 of mouse anti-BrdU antibody (#347580, BDBiosciences) and 1:500 rabbit anti-calbindin antibody (#13176, Cell Signaling Technologies).

Techniques: Phospho-proteomics, Expressing, Marker, Cell Differentiation

Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet: (A–F) Immunohistochemistry technique for capturing SNPH intrusion in PC dendrites. Shown is SNPH (green), Syt2 (red), and Calbindin (blue) labeling in 3.5-month-old WT (A, C, and E) and Shi (B, D, and F) mice. Scale bar, 10 μm. (G and H) High magnification of the maximum intensity projection image from the z stack through dendritic regions of WT (G) and Shi (H). (I and J) Orthogonal (slice) view of SNPH punctum (indicated by arrows in panels G and H) in the dendritic region from WT (I) and Shi (J) in x-z and y-z orientations, respectively. (K) Quantification of percentage area occupied by SNPH within the dendritic volume from 3 mice of each group. Data are shown as mean ± SEM. *p < 0.05. (L–N) Capturing SNPH intrusion by pre-tagging dendritic mitochondria in vivo using viral transduction. (L) Technique to selectively transduce PCs with AAV-Mito-mCherry. (M) Demonstration of successful pre-tagging of mitochondria in dendrites of a single PC by Calbindin staining. Scale bar, 10 μm. (N) Demonstration of how pre-tagged dendritic mitochondria in PCs are used to capture SNPH intrusion by 3D rotation. (O and P) Single dendritic tree in WT (O) or Shi (P) pre-tagged with Mito-mCherry (red) and SNPH intrusion (green) captured by co-rotation with Mito-mCherry. Merged images show the fraction of dendritic mitochondria anchored by intruded SNPH (yellow). (Q and R) Percentage of PCs with SNPH intrusions (Q) and percentage of SNPH bound to mitochondria per dendritic tree (R) from WT (n = 73) and Shi (n = 106) PCs. Data are shown as mean ± SEM. ***p < 0.005.

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Immunohistochemistry, Labeling, In Vivo, Transduction, Staining

Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet: (A and B) Representative images of lentivirally transduced GFP-SNPH (1–469) (A) and GFP-SNPH (B) in PCs of SNPH-KO mice injected with saline (no harmaline, vehicle only). (C-H) Effect of harmaline on GFP-SNPH (1–469)-transduced (C) and GFP-SNPH-transduced (F) PC dendrites. Degenerating dendrites in GFP-SNPH-transduced PCs can be seen in (F). Also shown is Calbindin labeling of GFP SNPH (1–469) (D) and GFP-SNPH (G) from (C) and (F). Merged images of GFP SNPH (1–469) and GFP-SNPH with Calbindin are shown in (E) and (H), respectively. (I–K) Representative image of a harmaline-induced degenerating PC (white arrow in I) transduced with GFP-SNPH. Calbindin staining from the same section is shown in (J), whereas a merged image is shown in (K). Scale bars, 20 μm. (L) Quantification of dendritic shrinkage in GFP-SNPH (1–469)- and GFP-SNPH-transduced PCs in the absence (n = 3 mice, vehicle only) or presence of harmaline (n = 5 mice). Data are shown as mean ± SEM. ***p < 0.001.

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Injection, Saline, Labeling, Transduction, Staining

Journal: Cell reports

Article Title: Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration

doi: 10.1016/j.celrep.2019.09.012

Figure Lengend Snippet:

Article Snippet: Polyclonal Calbindin , Boster , M03047–2.

Techniques: Virus, Plasmid Preparation, Recombinant, Software, Imaging

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Analysis Reveals an Essential Role of the Tyrosine Metabolic Pathway and Inflammation in Myopia-Induced Retinal Degeneration in Guinea Pigs

doi: 10.3390/ijms222212598

Figure Lengend Snippet: Primary and secondary antibodies used in immunofluorescence study.

Article Snippet: Calbindin , Rabbit , TA342845 , 1:50 , Origene , Horizontal cells.

Techniques: Immunofluorescence, Plasmid Preparation

Journal: Frontiers in Neuroscience

Article Title: Dystrophin Is Required for the Proper Timing in Retinal Histogenesis: A Thorough Investigation on the mdx Mouse Model of Duchenne Muscular Dystrophy

doi: 10.3389/fnins.2020.00760

Figure Lengend Snippet: Primary antibodies used for immunofluorescence.

Article Snippet: Rabbit anti-calbindin (HC) , 1:1000 , Merck Millipore (Billerica, MA, United States).

Techniques: Immunofluorescence

Journal: The Journal of Neuroscience

Article Title: Clustered Fine Compartmentalization of the Mouse Embryonic Cerebellar Cortex and Its Rearrangement into the Postnatal Striped Configuration

doi: 10.1523/JNEUROSCI.1710-12.2012

Figure Lengend Snippet: Antibody specification

Article Snippet: , Calbindin-D28k , Synthetic peptide derived from amino acids 185-199 of human Calbindin-D-28K , AnaSpec, rabbit polyclonal, Cat. # 53283, Lot # GL141 , 1:4000.

Techniques: Purification, Derivative Assay, Recombinant, Plasmid Preparation